The research approach(es) to be taken and methods to be followed (give reference to only those methods which are not already well known) in the sub-project to fill the gaps with logical justification and alternative routes if any, available. Based on this analysis clearly define the activities under each objective of the sub-project:  

Activities (Work plan)
Objective 1:

Isolation of bio-active compounds from five common weeds used as medicinal in Indian Systems of Medicine from rice, sugarcane and cotton based cropping systems of Andhra Pradesh

1. Identification of the five weed species on the basis of their demand as medicinal plants in the market; availability in commercially viable volumes and prior knowledge of their usages in curing diseases
2. Bio-assay guided isolation of bioactive compounds at different growth stages of the weed growth in different cropping systems and different seasons.

Objective 2:
Chemical identification of bio-active molecules and its derivatives

1. Elucidation of chemical structure of bio-active molecules using spectral data

Objective 3:
Validation of bio-active molecules

1. In-vitro and In-vivo validation of efficacy of bio-active compound (s) using appropriate test organisms

Objective 4:
Selection of highly bio-active rich genotypes of the five identified              

1. Selection of highly potent genotypes from each species and their chemotyping
2. Assessment of post-harvest handling techniques for preserving the medicinal properties of the identified weeds at the farm level